Tired of playing second banana to Bugs Bunny, Daffy Duck demands his own cartoon from Warner Bros., but is promptly fired.
Aspiring stuntman DJ Drake is asked to escort Daffy off the studio lot, but the ensuing chase leads to the Batmobile demolishing the studio water tower.
DJ is also fired in the process, and returns home with Daffy hitching a ride, discovering his father, action film star, Damian Drake, is actually a secret agent. Meanwhile, Bugs' routines fall apart without Daffy, so "Vice-President of Comedy," Kate Houghton, is sent to rehire Daffy or face being fired herself.
Damian instructs his son to travel to Las Vegas, find his associate Dusty Tails, and gain the mystical "blue monkey" diamond, but he is shortly thereafter captured by the Acme Corporation, led by the childish Mr. Bugs informs Kate of the situation, so they steal Damian's spy car, and pursue DJ and Daffy.
In Las Vegas, DJ and Daffy meet Dusty in a casino owned by Acme operative Yosemite Sam.
Dusty gives them a strange playing card, but when Sam attempts to kill them, they flee in the spy car with Bugs and Kate.
The spy car, which can also fly, crashes in the Nevada desert.
The group eventually stumbles upon Area 52, run by a woman called 'mother', who informs them of the situation, and plays a video recording, which reveals that Acme intends on using the blue monkey to transform mankind into monkeys to manufacture their products, before turning them back into human beings to purchase them.
Marvin the Martian, imprisoned in the facility, escapes and leads a group of fellow alien inmates to obtain the playing card, but the heroes escape.
Seeing that the card has Mona Lisa's face on it, the group conclude they must view the painting in the Louvre, located in Paris. Elmer is disintegrated by Bugs after jumping out of a pointillism artwork.
At the Louvre, they discover that the card contains a viewing window, and looking through it, the window reveals that the Mona Lisa has a map of Africa hidden beneath. Bugs and Daffy reunite with DJ and Kate, and they leave Paris.
Elmer Fudd appears, and, revealing himself as an Acme operative, chases Bugs and Daffy through the gallery for the card whilst Kate is kidnapped by Mr. DJ, Kate, Bugs, and Daffy travel to Africa, meeting Granny, Sylvester, and Tweety, who escort them to the ruins of a jungle temple where they find the blue monkey. Chairman, Smith, and the Tasmanian Devil in disguise. Chairman uses a disintegration gun to transport himself and the heroes to the Acme headquarters where he forces DJ to give him the diamond, when Damian is revealed to be his prisoner.